Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli.
Two methods were used in an attempt to increase the efficiency and strand selectivity of methyl-directed mismatch repair of bacteriophage lambda heteroduplexes in E. coli. Previous studies of such repair used lambda DNA that was only partially methylated as the source of methylated chains. Also, transfection was carried out in methylating strains. Either of these factors might have been respons...
متن کاملAntagonism of ultraviolet-light mutagenesis by the methyl-directed mismatch-repair system of Escherichia coli.
Previous studies have demonstrated that the Escherichia coli MutHLS mismatch-repair system can process UV-irradiated DNA in vivo and that the human MSH2.MSH6 mismatch-repair protein binds more strongly in vitro to photoproduct/base mismatches than to "matched" photoproducts in DNA. We tested the hypothesis that mismatch repair directed against incorrect bases opposite photoproducts might reduce...
متن کاملThe Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions.
A major role of the methyl-directed mismatch repair (MMR) system of Escherichia coli is to repair postreplicative errors. In this report, we provide evidence that MMR also acts on oxidized DNA, preventing mutagenesis. When cells deficient in MMR are grown anaerobically, spontaneous mutation frequencies are reduced compared with those of the same cells grown aerobically. In addition, we show tha...
متن کاملChromosomal directionality of DNA mismatch repair in Escherichia coli.
Defects in DNA mismatch repair (MMR) result in elevated mutagenesis and in cancer predisposition. This disease burden arises because MMR is required to correct errors made in the copying of DNA. MMR is bidirectional at the level of DNA strand polarity as it operates equally well in the 5' to 3' and the 3' to 5' directions. However, the directionality of MMR with respect to the chromosome, which...
متن کاملInteraction of MutS and Vsr: some dominant-negative mutS mutations that disable methyladenine-directed mismatch repair are active in very-short-patch repair.
In Escherichia coli and related bacteria, the very-short-patch (VSP) repair pathway uses an endonuclease, Vsr, to correct T-G mismatches that result from the deamination of 5-methylcytosines in DNA to C-G. The products of mutS and mutL, which are required for adenine methylation-directed mismatch repair (MMR), enhance VSP repair. Multicopy plasmids carrying mutS alleles that are dominant negati...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2002
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2002.02989.x